BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is ready to use solution.
Application Notes
The BCIP/NBT Liquid Substrate System offers a convenient, ready-to-use substrate solution for visualizing alkaline phosphatase activity in immunoblotting and immunohistology. Immunohistochemical staining with BCIP/NBT requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoblot | Immunohistochemistry
| SKU | 0898077-CF |
| Application Notes | The BCIP/NBT Liquid Substrate System offers a convenient, ready-to-use substrate solution for visualizing alkaline phosphatase activity in immunoblotting and immunohistology. Immunohistochemical staining with BCIP/NBT requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. |
| Base Catalog Number | 980771,980772 |
| Concentration | BCIP: 0.692 mmol/liter; NBT: 0.734 mmol/liter |
| Formulation Details | Liquid |
| pH | 9.8 [+/- 0.1] |
| Preparation Method | BCIP/NBT [5-bromo-4-chloro-3-indolyl-phosphate p-nitro blue tetrazolium chloride] is in a proprietary buffer and enhancer |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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