Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.
Application Notes
β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used as reporter gene | Enzymatic hydrolysis of glucuronides
| SKU | 02152284-CF |
| Alternate Names | β-D-Glucuronide glucuronosohydrolase |
| Application Notes | β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression. |
| Base Catalog Number | 152284 |
| Biochemical Physiological Actions | β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.β-Glucuronidase from Helix pomatia is a mixture of enzymes. Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone |
| Format | Supplied in 65% Saturated Ammonium Sulfate Solution. |
| Hazard Statements | H317-H334 |
| Personal Protective Equipment | Breathing apparatus,gloves,Safety glasses |
| RTECS Number | LZ8990000 |
| Safety Symbol | GHS08 |
| Source | Helix pomatia |
| Specific Activity | Sulfatase Activity |
| Sterility | ≤5000 u/ml |
| Storage | BIOCHEMICALS |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | Unit definition: amount that liberates 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 5.0 and 37°C. |
![MP COA [152284] lot ID: M2805](https://www.mpbio.com/static/version1780565991/frontend/Mpbio/luma/en_US/images/document.png "MP COA [152284] lot ID: M2805")
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