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Phosphate Buffered Saline (PBS), Dulbecco's formula, powder, w/o Ca, Mg
Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
Application Notes
Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Mammalian Cell Culture
SKU | 0917604-CF |
Alternate Names | PBS Powder; DPBS |
Application Notes | Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. |
Base Catalog Number | 1760420,1760422,1760426 |
Format | Powder |
Formulation Details | Powder | Inorganic Salts: Potassium Chloride [KCl]: 200.0000 mg/L; Potassium Phosphate Monobasic [KH<sub>2</sub>PO<sub>4</sub>]: 200.0000 mg/L; Sodium Chloride [NaCl]: 8000.0000 mg/L; Sodium Phosphate Dibasic [Na<sub>2</sub>HPO<sub>4</sub>]: 1150.0000 mg/L |
Media Type | Dulbecco PBS |
pH | 7.4 ± 0.1 |
Solubility | Dissolve one packet of powder in 1 L of distilled water. The pH will be in the range of 7.3 - 7.5. If desired, add sodium bicarbonate. The pH can be adjusted as desired using 1N HCl or 1N NaOH. Sterile filter, if desired. Store liquid at 2 - 8 °C. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |