Phosphate Buffered Saline (PBS), Dulbecco's formula, powder, w/o Ca, Mg

Product Description

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Application Notes

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Mammalian Cell Culture

Specifications

SKU	0917604-CF
Alternate Names	PBS Powder; DPBS
Application Notes	Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
Base Catalog Number	1760420,1760422,1760426
Format	Powder
Formulation Details	Powder \| Inorganic Salts: Potassium Chloride [KCl]: 200.0000 mg/L; Potassium Phosphate Monobasic [KH<sub>2</sub>PO<sub>4</sub>]: 200.0000 mg/L; Sodium Chloride [NaCl]: 8000.0000 mg/L; Sodium Phosphate Dibasic [Na<sub>2</sub>HPO<sub>4</sub>]: 1150.0000 mg/L
Media Type	Dulbecco PBS
pH	7.4 ± 0.1
Solubility	Dissolve one packet of powder in 1 L of distilled water. The pH will be in the range of 7.3 - 7.5. If desired, add sodium bicarbonate. The pH can be adjusted as desired using 1N HCl or 1N NaOH. Sterile filter, if desired. Store liquid at 2 - 8 °C.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Documents

Datasheet

DS [0917604]

Citations