Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to bovine IgG (whole molecule) and buffer salts.
Application Notes
Immunoblotting
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
| SKU | 0855280 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Immunoblotting |
| Base Catalog Number | 55280 |
| Concentration | Total Protein: 15.5 mg/ml F/P Ratio: 4.2 mol/mol Antibody Protein: 5 mg/ml |
| Conjugate | FITC |
| Format | lyophilized |
| Grade | Molecular Grade |
| Host | Rabbit |
| Pack Size | 2 mL |
| Solubility | Soluble in water |
| Species Reactivity | Bovine |
| Specificity | The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. This product shows reactivity to bovine IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |