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Anti-human IgA (Alpha Chain) affinity-purified goat antibody, 1 mg

Anti-human IgA (Alpha Chain) affinity-purified goat antibody, 1 mg

Affinity purified goat antibody to human IgA (α-chain) and buffer salts.

SKU: 0855068

MDL Number:
MFCD00162412
Packaging size:
1 mg
Product Description

Product is goat affinity purified antibody to human IgA (α-chain) and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay. It may be used to locate and identify the α-chain of human IgA by immunocytochemical and immunohistochemical (IHC) techniques.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Ouchterlony double diffusion | Immunohistochemistry

Specifications
SKU 0855068
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay. It may be used to locate and identify the α-chain of human IgA by immunocytochemical and immunohistochemical (IHC) techniques.
Base Catalog Number 55068
Concentration Total Protein: 1.1 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Goat
Immunogen Purified human IgA
Pack Size 1 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Purity Affinity Purified
Solubility Soluble in Water
Species Reactivity Human (Alpha Chain)
Specificity It shows reactivity to human IgA; cross-reactivity to other species may exist. Antibody activity to IgG; IgM; or non-immunoglobulin serum proteins is not present.
Sterility Filtered through a .22 µm filter
Typical Working Concentration Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1: 2,000 for ELISA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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