Urine Collection and Preservation Kit

Urine contains both nucleated cells and cell-free DNA, which are unstable and prone to rapid lysis or degradation upon sample collection. In addition, exfoliated nucleated cells such as normal epithelial cells or cancer-derived cells release genomic DNA which leads to dilution of cell-free DNA. To prevent nucleated cells and the cell-free DNA from degradation during storage and shipping, efficient stabilization methods are needed. 

Urine Preservation Solution offers simultaneous stabilization of cell-free DNA, genomic DNA, and cells in urine up to 30 days at ambient temperature. The Urine Preservation Solution can be added directly to urine samples after collection, without the need to separate nucleated cells and cell-free DNA by centrifugation.

Figure 1: Performance of the Urine Preservation Solution in urinary cell stabilization. Urine samples from (a) one female donor and (b) one male donor were treated with either the Urine Preservation Solution or Competitor N and stored at ambient temperature. Genomic DNA was extracted using the SPINeasy DNA Kit for Urine on Days 0, 7, 10, 30, and 45. Quantitative PCR (qPCR) was performed using 4 μL of gDNA per reaction. Threshold cycle (Ct) values were compared. Targets were amplified using SYBR Green technology.

Figure 2: Performance of the Urine Preservation Solution in stabilizing cell-free DNA (cfDNA) in urine. Urine samples from (a) one female donor and (b) one male donor were treated with the Urine Preservation Solution, Competitor N, or Competitor Z and stored at ambient temperature. Cell-free DNA was extracted using the SPINeasy DNA Kit for Urine on Days 0, 7, 10, 30, and 45. Quantitative PCR (qPCR) was performed using 4 μL of cfDNA per reaction. Threshold cycle (Ct) values were compared. Targets were amplified using SYBR Green technology.

Application Notes

The Urine Collection and Preservation Kit is for the stabilization of gDNA, cell-free DNA, and cells in urine samples.

Usage Statement

Research Use Only (RUO)