Orange G, sodium salt

Product Description

Orange G Sodium Salt

Application Notes

Orange G is used primarily as a histological stain. It is a pail dye that is used for faint background staining. It is used as a counterstain for a number of nuclear stains including hematoxylin, safranin O, crystal violet, methyl green and basic fuchsin. Also used as a plasma stain. Orange G is a major component in the Papanicolaou stain, which is used in cervical cytology. The compound has been used to stain cells secreting growth hormone in the anterior pituitary and stain the mature bone orange. Orange G has also been incorporated in the following: Mallory's connective tissue stain,Flemming's triple stain on paraffin sections of onion root, Kreyberg's method for keratin and mucus and prostatic carcinoma cells, as a counterstain following Heidenhain's iron hematoxylin on paraffin sections and in the Wilson-Ezrin method for pituitary acidophils. It is also used as a collagen stain for connective tissue. Used with Orcein, Alizarin Blue 2B, and Fast Green FCF in Kornhauser′s quadruple stain for most elementary structures of tissues.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Histological stain | Used in Mallory's connective tissue stain | Kreyberg's method for keratin and mucus and prostatic carcinoma cells

Specifications

SKU	02151787-CF
Alternate Names	Acid orange 10; C.I. 16230; Wool Orange 2G; 1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt; 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt
Application Notes	Orange G is used primarily as a histological stain. It is a pail dye that is used for faint background staining. It is used as a counterstain for a number of nuclear stains including hematoxylin, safranin O, crystal violet, methyl green and basic fuchsin. Also used as a plasma stain. Orange G is a major component in the Papanicolaou stain, which is used in cervical cytology. The compound has been used to stain cells secreting growth hormone in the anterior pituitary and stain the mature bone orange. Orange G has also been incorporated in the following: Mallory's connective tissue stain,Flemming's triple stain on paraffin sections of onion root, Kreyberg's method for keratin and mucus and prostatic carcinoma cells, as a counterstain following Heidenhain's iron hematoxylin on paraffin sections and in the Wilson-Ezrin method for pituitary acidophils. It is also used as a collagen stain for connective tissue. Used with Orcein, Alizarin Blue 2B, and Fast Green FCF in Kornhauser′s quadruple stain for most elementary structures of tissues.
Auto Ignition	260-371Â°C
Base Catalog Number	151787
Beilstein Registry Number	4120705
Boiling Point	218-643Â°C
CAS #	1936-15-8
Color Index	16230
Density	g/cmÂ³
EC Number	217-705-6
Flash Point	>115Â°C o.c.
Format	Crystalline powder
Melting Point	572 to 707Â° F (decomposes) (NTP, 1992)
Molecular Formula	C16H10N2O7S2Na2
Molecular Weight	452.363 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
RTECS Number	QJ6500000
Solubility	50 to 100 mg/mL at 74.3Â° F (NTP, 1992)
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	λ max (water): 475 - 481 nm
Vapor Pressure	<h5 class="minor-section-title">Vapour pressure</h5>at 20Â°C: negligible