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Ribonuclease A (bovine)

Ribonuclease A (bovine)

SKU: 02101076-CF

Pancreatic Ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′ pyrimidinooligonucleotidohydrolase
CAS Number:
Molecular Weight:
13700 (based on Amino acid sequence)
EC Number:
MDL Number:
SKU 02101076-CF
Alternate Names Pancreatic Ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′ pyrimidinooligonucleotidohydrolase
Application Notes Ribonuclease A, and to a greater extent its oligomers and some homologs (such as onconase from frogs), have cytotoxic and cytostatic effects, particularly on cancer cells.This attribute has led to the development of onconase as a cancer therapeutic.
Base Catalog Number 101076
Biochemical Physiological Actions Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2'-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
CAS # 9001-99-4
EC Number 232-646-6
Extinction Coefficient 7.3 (280 nm) (Lit.)
Isoelectric Point pH 9.45 (Lit.)
Molecular Weight 13700 (based on Amino acid sequence)
Personal Protective Equipment Eyeshields, Gloves, Repirator Filter
pH Optimum pH: 7.0 - 7.5
RTECS Number RF0760000
Solubility Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source Bovine Pancreas
Specific Activity ≥50 Kunitz u/mg
Specificity The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Unit Definition That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity at 25 °C and pH 5.0.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Certificate of Analysis (COA)