Glucose Oxidase
Application Notes
Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction. Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Enzymes | Inhibitors | Substrates
| SKU | 0219519650 |
| Alternate Names | β-D-Glucose:oxygen 1-oxidoreductase; G.Od.; Gox |
| Application Notes | Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction. Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors. |
| Base Catalog Number | 19519650 |
| Biochemical Physiological Actions | Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor. |
| CAS # | 9001-37-0 |
| EC Number | 232-601-0 |
| Extinction Coefficient | 16.7 (280 nm)(Lit.) |
| Format | Lyophilized powder |
| Hazard Statements | H334 |
| Isoelectric Point | 4.2 (Lit.) |
| Melting Point | 83 °C |
| Molecular Weight | 180.156 g/mol |
| Pack Size | 50 KU |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| RTECS Number | RQ8452000 |
| Safety Symbol | GHS08 |
| Solubility | Water Solubility1200000 mg/L (at 30 °C) |
| Source | Aspergillus niger |
| Specific Activity | >100 u/mg material |
| Unit Definition | One unit of glucose oxidase is the activity which causes the liberation of 1 micromole of glucose per minute at 25 °C and pH 7.0 under the specified conditions. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [195196] lot ID: M5683](https://www.mpbio.com/static/version1744790984/frontend/Mpbio/default/en_US/images/document.png "MP COA [195196] lot ID: M5683")