5-Bromo-4-chloro-3-indolyl phosphate, disodium salt

Name: 5-Bromo-4-chloro-3-indolyl phosphate, disodium salt
SKU: 02150042-CF
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SKU: 02150042-CF

Key features and details

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

Synonyms

BCIP; X-phosphate disodium salt

CAS Number:

102185-33-1

Molecular Formula:

C8H4BrClNO4PNa2

Molecular Weight:

370.43 g/mol

MDL Number:

MFCD00036757

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Product Description

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

Application Notes

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Substrate for alkaline phosphatase

Specifications

SKU	02150042-CF
Alternate Names	BCIP; X-phosphate disodium salt
Applications	Substrate for alkaline phosphatase
Application Notes	For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
Base Catalog Number	150042
CAS #	102185-33-1
Format	Powder
Molecular Formula	C8H4BrClNO4PNa2
Molecular Weight	370.43 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Purity	≥99%
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	λ max (water): 291 ± 3 nm