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3,3' Diaminobenzidine tetrahydrochloride, 100 mg

3,3' Diaminobenzidine tetrahydrochloride, 100 mg

SKU: 0215082680

CAS Number:
Molecular Formula:
Molecular Weight:
Beilstein Registry Number:
EC Number:
MDL Number:
Packaging size:
100 mg
SKU 0215082680
Alternate Names DAB
Application Notes 3,3′-Diaminobenzidine tetrahydrochloride (DAB) is a horseradish peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble, brown end product, which can be observed visually.
Base Catalog Number 15082680
Beilstein Registry Number 1212988
CAS # 7411-49-6
EC Number 231-018-9
Hazard Statements H341-H350
Melting Point > 300 °C (Lit.)
Molecular Formula C12H14N4*4HCl
Molecular Weight 360.10
Pack Size 100 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Preparation Method Typical Preparation of solutions for Peroxidase Detection : Dissolve 12 mg of DAB in 1.0 mL 50 mM Tris buffer, pH 7.3 (or inject 8.3 mL of buffer into serum vials if using #150826). Working size aliquots may be stored for 6 months at -20 °C. The stock solution is diluted 1:20 with 50 mM Tris buffer pH 7.3 just before developing the immunoblot. To 10 mL add 10 uL 30% hydrogen peroxide. Add 10 mL of this substrate solution per 15 x 15 cm2 membrane. Develop the blot at room temperature with agitation until the bands are suitably dark. This typically requires 1-5 min. Stop the reaction by rinsing away the substrate with PBS.
RTECS Number DV8753000
Safety Symbol GHS08
Solubility Easily soluble in water. The aqueous solution is unstable and can be used only for a few days even if it is kept in a refrigerator. 1% aqueous solution is dark and turns reddish when exposed to light.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.