Purified Mouse Myeloma Protein IgG1 (Kappa) MOPC 21

Purified Mouse Myeloma Protein IgG1 (Kappa) MOPC 21

Synonyms Mouse IgG1/k

MDL Number: MFCD00164798

Packaging size: 1 mg

SKU 0850327
Price:
$190.60
In Stock
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Description

Product Description

Product is purified mouse IgG1 (K) and buffer. Mouse IgG1 (K) is purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Control

Specifications
SKU 0850327
Alternate Names Mouse IgG1/k
Analysis Note The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG1. The product is tested for purity at 1.0 mg/mL using immunoelectrophoresis. The product is mouse IgG1; no trace of IgG2a, IgG2b, IgG3, IgA, IgM or non-immunoglobulin serum proteins is detected.
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Clone Name MOPC 21
Concentration Total Protein: 1.2 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Isotype IgG
Applications Immunoassays | ELISA | Control
MDL # MFCD00164798
Pack Size 1 mg
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Physical Appearance Puried product in buffer
Preparation Method Mouse IgG1 (K) is purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Presentation Purified proteins are dialyzed into 0.02M Tris; 0.14M sodium chloride; pH 7.4.
Purity Affinity Purified
Recommended Replacement For 864335
Research Areas Used as positive control in antibody identification screening techniques
ELISA coating antigen and control
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG1. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG1; no trace of IgG2a; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Sterility Filtered through a .22 µm filter
Storage and Handling Store unopened product at -70°C. Store opened material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -70°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.