Zymolyase 100T (100Ku/g)

Zymolyase 100T (100Ku/g)

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SKU 083209-CF
Price:
$708.65
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Description

Product Description

Zymolyase-100T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is lyophilized enzyme partially purified by affinity chromatography and contains 100,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells in ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.

Application Notes

Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-1T has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration.

Key Applications

Yeast Cell Culture | Used to lyse cell wall of yeast

Specifications
SKU 083209-CF
Application Notes Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-1T has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration.
Formulation Details Lyophilized
Applications Yeast Cell Culture | Used to lyse cell wall of yeast
pH Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells; pH 6.5, 45 °C for hydrolysis of yeast glucan; pH Range: Stable through pH ranges 5-10
Physical Appearance Lyophilized powder
Presentation Lyophilized powder
Protein or Enzyme Type Yeast Lytic Enzymes
Research Areas Cell Structure
Source Arthrobacter luteus
Specific Activity minimum of 100,000 units/g
Specificity The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwannimomyces and others.
Storage and Handling Store at 2 - 8°C in the lyophilized state. Approximately 90% of activity is lost when stored at 30°C for 3 months.
Trade Name Immuno™
Unit Definition The amount of activity causing a decrease in absorbance at 800 nm of 30%.