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Trypsin inhibitor, from soybean, ≥10,000 BAEE unit

Trypsin inhibitor, from soybean, ≥10,000 BAEE unit

Synonyms Kunitz Trypsin Inhibitor; Tia1; STI; SBT1

CAS Number: 9035-81-8

EC Number: 232-906-9 MDL Number: MFCD00082103

SKU 02101113-CF
$46.40
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Description

Product Description

Trypsin Inhibitor

Application Notes

After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell culture

Specifications
SKU 02101113-CF
Alternate Names Kunitz Trypsin Inhibitor; Tia1; STI; SBT1
Application Notes After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.
Base Catalog Number 101113
Biochemical Physiological Actions Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
CAS # 9035-81-8
EC Number 232-906-9
Extinction Coefficient E1% = 9.94 (280 nm, pH 7.6 buffer) (Lit.)
Format Powder
Hazard Statements H317-H334
Isoelectric Point 4.5 (Lit.)
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Safety Symbol GHS08
Source Soybean
Specific Activity ≥10,000 BAEE units of inhibition/mg material
Unit Definition One unit will inhibit one trypsin BAEE unit. One trypsin unit causes an increase of absorbance at 253 nm of 0.001 per minute at 25 °C.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.