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Thymidine, cell culture reagent, >99%
Thymidine is a nucleoside composed of deoxyribose (a pentose sugar) joined to the pyrimidine base thymine.Deoxythymidine can be phosphorylated with one, two or three phosphoric acid groups, creating respectively dTMP, dTDP or dTTP (deoxythymidine mono- di- or triphosphate.It exists in solid form as small white crystals or white crystalline powder.The stability of deoxythymidine under standard temperature and pressure (STP) is very high.Thymidine is listed as a chemical teratogen.
Mainly used in forming hybrid cell lines wherein cells are incubated in HAT medium(hypoxanthine-aminopterin-thymidine medium) to give rise to monoclonal antibodies. Use in HT and HAT cocktails for hybridoma fusion, selection and cloning.
|Alternate Names||Thymine-2-deoxyriboside; Deoxythymidine; DT; DTHYD;1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil; 1-(2-Deoxy-β-D-ribofuranosyl)thymine; 2′-Deoxythymidine; Thymine deoxyriboside; Dt|
|Application Notes||Mainly used in forming hybrid cell lines wherein cells are incubated in HAT medium(hypoxanthine-aminopterin-thymidine medium) to give rise to monoclonal antibodies. Use in HT and HAT cocktails for hybridoma fusion, selection and cloning.|
|Base Catalog Number||194754|
|Beilstein Registry Number||89285|
|Grade||Cell Culture Grade|
|Melting Point||185 - 191°C|
|Optical Rotation||(c=1, water)|
|Particle Size||+17.5 - +19.5°|
|pH||Eyeshields, Gloves,Respirator filter|
|Source||Soluble in water; methanol; hot alcohol; hot acetone; hot ethyl acetate pyridine; glacial acetic acid and HCl (20 mg/mL-clear; colorless solution); sparingly soluble in hot chloroform.|