Enjoy 30% off select life science products with free shipping and giveaways with promo code: RESEARCH30 Offer ends October 2, 2020.
Tetracyline inhibits gram-positive, gram-negative and mycoplasma. It is used in tetracycline controlled gene expression systems (gene switches) such as the tet-on and tet-off systems. It is also used to select tetracyline resistant colonies in cell culture.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Antibiotic | Used in tetracycline controlled gene expression systems | Used to select tetracyline resistant colonies
|Alternate Names||Abramycin; Bristacycline; Democracin; Sanclomycine; Tetraverine; [4S-(4a, 4aa, 5aa, 6b, 12aa)]- 4-(Dimethylamino)- 1, 4, 4a, 5, 5a, 6-11, 12a- octahydro-3, 6, 10, 12, 12a-pentahydroxy- 6- methyl-1, 11-dioxo-2-naphthacenecarboxamide; deschlorobiomycin; tsiklomitsin; Abricycline; Bio-Tetra; Cyclomycin; Dumocyclin; Tetradecin.|
|Application Notes||Tetracyline inhibits gram-positive, gram-negative and mycoplasma. It is used in tetracycline controlled gene expression systems (gene switches) such as the tet-on and tet-off systems. It is also used to select tetracyline resistant colonies in cell culture.|
|Base Catalog Number||103012|
|Beilstein Registry Number||2230417|
|Biochemical Physiological Actions||Mode of Action: Tetracycline passively diffuses through proin channels in the cell membrane, binding to 30S ribosomes and inhibits protein synthesis by preventing access of aminoacyl tRNA to the acceptor site on the mRNA-ribosome complex. It also binds to the bacterial 50S ribosomal subunit, altering the membrane and causing intracellular components to leak from bacterial cells. The inhibitory effects can be reversed by washing, suggesting that it is the reversibly bound antibiotic, and not the irreversibly bound drug, that is responsible for antibacterial action.Mode of Resistance: The effects are inactivated via a loss of cell wall permeability.Antimicrobial spectrum: Includes a wide range of antimicrobial activity against gram-positive and gram-negative bacteria.|
|Melting Point||417Â° F (decomposes) (NTP, 1992)|
|Molecular Weight||444.44 g/mol|
|Optical Rotation||[α]20/D −230±5°, c = 1% in methanol|
|Personal Protective Equipment||Dust mask, Eyeshields, Gloves|
|pH||3.0-7.0 /saturated solution/|
|pKa||8.3, 10.2(50% aq DMF)(lit.)|
|Solubility||50 to 100 mg/mL at 72Â° F (NTP, 1992)|
|Typical Working Concentration||10 ug/mL|
|UV Visible Absorbance||λmax (0.1N HCl) 217-220, 268-271, 355-356 nm|