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Saponin

Saponin

Synonyms Sapogenin Glycosides

CAS Number: 8047-15-2

EC Number: 232-462-6 MDL Number: MFCD00081981

SKU 02102855-CF
$38.05
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Description

Product Description

Saponin

Application Notes

1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Hemolyze erythrocytes | Permeabilize cultured human intestinal epithelial cells and paraformaldehyde-saponin- fixed human fibroblasts | Surface-active agent

Specifications
SKU 02102855-CF
Alternate Names Sapogenin Glycosides
Application Notes 1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines.
Base Catalog Number 102855
Biochemical Physiological Actions Potent hemolytic when injected i.v.; surfactant that enhances penetration of proteins and other macromolecules through cell membranes
CAS # 8047-15-2
EC Number 232-462-6
Format Powder
Grade Practical Grade
Hazard Statements H319-H335
Personal Protective Equipment Dust mask, Eyeshields, Gloves
RTECS Number VQ1400000
Safety Symbol GHS07
Source Plant
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.