Recombinant SARS-CoV-2 Spike S1 (B.1.1.529/Omicron) Protein (His tag)
Product Description
MP Biomedicals recombinant SARS-CoV-2 Spike S1 (B.1.1.529/Omicron) Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Gln14-Arg683 (A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)) of SARS-CoV-2 Spike S1 (B.1.1.529/Omicron) (Accession #QHO60594.1) fused with His tag at the C-terminus.
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, SPR, SDS-PAGE, Western Blotting, Immunoprecipitation, Immune Blocking, Binding Assay
Specifications
| SKU | 08L100015 |
| Alternate Names | S1 protein; Spike glycoprotein subunit1; Omicron variant S1 protein |
| Bioreactivity | Immobilized Human ACE2 at 5 μg/mL (100 μL/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (B.1.1.529/Omicron) with the EC50 of 1.5 μg/mL determined by ELISA. |
| Concentration | 1 mg/mL if dissolve in 100 µL of ddH20 or biological buffer |
| Conjugate | Unconjugated |
| Endotoxin | < 1 EU/μg of the protein by LAL method |
| Formulation Details | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.3-7.4. |
| Grade | Molecular Grade |
| Host | Expi293 cells |
| Pack Size | 100 μg |
| Protein Tag | His tag |
| Purity | >97% by SDS-PAGE; > 95% by HPLC |
| Solubility | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Addition of a carrier protein (such as 0.05-0.1% BSA) is recommended for long-term storage. |
| Species Reactivity | Human, Mouse, Rat |
| Storage | -20° |
| Uniprot Number | QHO60594.1 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Safety Data Sheets
English (United States)
Brochures
Brochures
English (United States)