Recombinant SARS-CoV-2 Spike RBD (B.1.1.529/Omicron) Protein (His tag)
SKU: 08L100017
Recombinant SARS-CoV-2 Spike RBD (B.1.1.529/Omicron) is produced by Expi293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541 (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)) of SARS-COV-2 Spike RBD (B.1.1.529/Omicron) (Accession #QHO60594.1) fused with His tag at the C-terminus.
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, SPR, SDS-PAGE, Western Blotting, Immunoprecipitation, Immune Blocking, Binding Assay
| SKU | 08L100017 |
| Alternate Names | S1 protein; Spike glycoprotein subunit1; Omicron variant S1 protein |
| Bioreactivity | Immobilized SARS-CoV-2 Spike RBD (B.1.1.529/Omicron) at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human ACE2 with the EC50 of 7.5 ng/mL determined by ELISA. |
| Concentration | 1 mg/mL if dissolve in 100 µL of ddH20 or biological buffer |
| Conjugate | Unconjugated |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method |
| Formulation Details | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.3-7.4. |
| Grade | Molecular Grade |
| Host | Expi293 cells |
| Pack Size | 100 μg |
| Preparation Method | Affinity Chromatography |
| Protein Tag | His Tag |
| Purity | >97% by SDS-PAGE; > 95% by HPLC |
| Species Reactivity | Human, Mouse, Rat |
| Storage | -20° |
| Uniprot Number | QHO60594.1 |
| Usage Statement | Research use only, not for direct human use. |