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P-Nitrophenyl phosphate di(tris) salt

P-Nitrophenyl phosphate di(tris) salt

Synonyms 4-Nitrophenyl phosphate di(tris) salt; 4-Nitrophenyl phosphate bis(tris) salt; pNPP

CAS Number: 68189-42-4 Molecular Formula: C14H28N3O12P Molecular Weight: 461.361 g/mol

Beilstein Registry Number: 1578142 EC Number: 269-198-6 MDL Number: MFCD00037009

SKU 02151766-CF
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Description

Product Description

P-Nitrophenyl Phosphate Di(Tris) Salt

Application Notes

p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Substrate for assay of alkaline phosphatase

Specifications
SKU 02151766-CF
Alternate Names 4-Nitrophenyl phosphate di(tris) salt; 4-Nitrophenyl phosphate bis(tris) salt; pNPP
Application Notes p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.
Base Catalog Number 151766
Beilstein Registry Number 1578142
CAS # 68189-42-4
EC Number 269-198-6
Format Powder
Molecular Formula C14H28N3O12P
Molecular Weight 461.361 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance λ max (water) 219-223, 307-311 nm