P-Nitrophenyl phosphate di(tris) salt

Name: P-Nitrophenyl phosphate di(tris) salt
SKU: 02151766-CF
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SKU: 02151766-CF

Key features and details

P-Nitrophenyl Phosphate Di(Tris) Salt

Synonyms

4-Nitrophenyl phosphate di(tris) salt; 4-Nitrophenyl phosphate bis(tris) salt; pNPP

CAS Number:

68189-42-4

Molecular Formula:

C14H28N3O12P

Molecular Weight:

461.361 g/mol

Beilstein Registry Number:

1578142

EC Number:

269-198-6

MDL Number:

MFCD00037009

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Product Description

P-Nitrophenyl Phosphate Di(Tris) Salt

Application Notes

p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Substrate for assay of alkaline phosphatase

Specifications

SKU	02151766-CF
Alternate Names	4-Nitrophenyl phosphate di(tris) salt; 4-Nitrophenyl phosphate bis(tris) salt; pNPP
Application Notes	p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.
Base Catalog Number	151766
Beilstein Registry Number	1578142
CAS #	68189-42-4
EC Number	269-198-6
Format	Powder
Molecular Formula	C14H28N3O12P
Molecular Weight	461.361 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	λ max (water) 219-223, 307-311 nm