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o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
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Substrate for β-galactosidase
|Alternate Names||o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG|
|Application Notes||o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.|
|Base Catalog Number||102473|
|Beilstein Registry Number||92207|
|Biochemical Physiological Actions||β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.|
|Optical Rotation||-66 ° ± 7 ° (c=1, H2O)|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|UV Visible Absorbance||262 ± 3 nm λ max (H2O)|