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O-Nitrophenyl-β-D-galactopyranoside

O-Nitrophenyl-β-D-galactopyranoside

Synonyms o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG

CAS Number: 369-07-3 Molecular Formula: C12H15NO8

Beilstein Registry Number: 92207 EC Number: 206-716-1 MDL Number: MFCD00063255

SKU 02102473-CF
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Description

Product Description

O-Nitrophenyl-Β-D-Galactopyranoside

Application Notes

o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Substrate for β-galactosidase

Specifications
SKU 02102473-CF
Alternate Names o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG
Application Notes o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
Base Catalog Number 102473
Beilstein Registry Number 92207
Biochemical Physiological Actions β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
CAS # 369-07-3
EC Number 206-716-1
Format Crystalline
Molecular Formula C12H15NO8
Optical Rotation -66 ° ± 7 ° (c=1, H2O)
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 262 ± 3 nm λ max (H2O)