Mouse anti-GAPDH mAb (High Potency)
SKU: 08L100030
MP Biomedicals GAPDH Mouse mAb (High Potency) offers high specificity and potency and consistency against GAPDH expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) assays.
Usage Statement
Research use only, not for direct human use.
Key Applications
Western blotting (WB), Immunohistochemistry (IHC) and Immunofluorescence (IF) assays
| SKU | 08L100030 |
| Alternate Names | G3PD; GAPD; HEL-S-162eP; GAPDH |
| Antibody Type | Monoclonal Antibody |
| Background_information | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophages. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
| Base Catalog Number | L100030 |
| Bioreactivity | This GAPDH Mouse mAb (High Potency) offers high specificity and potency and consistency against GAPDH expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) assays. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Formulation Details | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
| Grade | Molecular Grade |
| Host | Mouse |
| Immunogen | Immunogen is a full-length recombinant protein of human GAPDH |
| Isotype | IgG |
| Molecular Weight | Observed at 36KDa |
| Pack Size | 100 µL |
| Preparation Method | Affinity purification |
| Solubility | Dissolves in Tris or PBS buffer immediately |
| Species Reactivity | Human, Mouse, Rat |
| Specificity | High specificity against antigens |
| Storage | -20° |
| Typical Working Concentration | WB: 1:50000 - 1:200000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200 |
| Uniprot Number | P04406 |
| Usage Statement | Research use only, not for direct human use. |