MagBeads FastRNA Kit for Virus (Ready-to-use for MPure-32™)

The study of viruses is critical for understanding their impact on human health and disease. In order to conduct research on these infectious agents, scientists rely on high-quality isolation and purification of viral RNA. One of the key challenges in studying viruses is the isolation and purification of high-quality viral RNA, which is essential for downstream molecular analyses. This process is often time-consuming, tedious, and requires a high level of expertise. To overcome these challenges, the MagBeads FastRNA Kit for Virus has been designed to provide researchers with a fast, efficient, and reliable method for extracting viral RNA from various sample types.

 

The MagBeads FastRNA Kit for Virus is specifically optimized for viral RNA extraction, providing high yield and purity. The extracted RNA can be used for downstream applications such as RT-PCR, qPCR, and sequencing; hence, enabling researchers to better understand viral infections and develop effective treatments. With its simple protocol and fast processing time, the MagBeads FastRNA Kit for Virus can be an essential tool for isolation of viral RNA from a wide variety of viruses. The intended use of this kit is for general utilization; its performance may exhibit variability depending on the type of virus sample.

 

Performance

MagBeads FastRNA Kit for Virus is a reliable solution for isolation of viral RNA from a variety of cell-free fluid samples including plasma, PBS, saliva, swabs, cell-culture media, etc. Results demonstrated that RNA extracted using MagBeads FastRNA Kit for Virus has been free of contaminants that could interfere with downstream applications. High-quality RNA obtained using this kit is an ideal starting material for real-time PCR, which ensures accurate and reproducible results.  

 

 

Figure 1: Real time RT-PCR of 10¹ to 10⁵ copies of Quantitative Genomic RNA from Influenza B virus (ATCC VR-1883DQ). High RNA recovery is achieved, with R²= 0.9987 for RNA extracted using MagBeads FastRNA Kit for Virus.

 

 Figure 2: Real time RT-PCR of RNA extracted from various virus-spiked samples. The same amount of Influenza B virus is spiked into 100 µL or 200 µL of various samples, followed by RNA extraction using MagBeads FastRNA Kit for Virus. Similar Ct is obtained for the different samples. This kit is suitable for virus RNA extraction using samples from cell culture media, swabs, and bodily fluids.

Application Notes

MagBeads FastRNA Kit for Virus (Ready-to-use for MPure-32™) is specifically optimized for viral RNA extraction, providing high yields and purity. The extracted RNA can be used for downstream applications such as RT-PCR, qPCR, and sequencing; hence, enabling researchers to better understand viral infections and develop effective treatments.

Usage Statement

Research Use Only (RUO).

Key Applications

Genomic, Molecular Biology, PCR, qPCR, Virus, RNA