MagBeads FastDNA™ Kit for Blood (Ready-to-Use for MPure-32)

Magbeads FastDNA^® Kit for Blood is based on the purification method of high binding magnetic particles. The sample is lysed and digested. DNA is released into the lysate. After addition of magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove the proteins and impurities, washed with ethanol to remove salts, and finally the DNA was eluted with Elution Buffer.

Figure 1: Extraction Efficiency of MagBeads FastDNA Kit for Blood Using Manual Method and MPure™ System. The MagBeads FastDNA Kit for Blood showed high-quality and consistent gDNA extraction, with comparable purity and yield across both manual and MPure™ extraction methods 

Figure 2: (A) gDNA was extracted from blood samples under different conditions and analyzed on a 1% agarose gel. Lanes are labeled as follows: "Short" (~1106 bp), "Medium" (~1424 bp), "Long" (~5778 bp), and "NTC" (Negative Template Control, no visible band). (B) Long fragments of the human BRCA gene were amplified via Endpoint PCR. DNA extracted with the MagBeads FastDNA Kit (MP) successfully produced the long fragment gene product, while DNA extracted with the Competitor T kit failed to amplify the long fragment. 

Figure 3: DNA Integrity Analysis of Blood gDNA Extracted with MP MagBeads FastDNA Kit for Blood. Genomic DNA was extracted from 200 µL blood using the MP MagBeads FastDNA Kit and compared to other competitor kits. The MP MagBeads kit yielded DNA with superior integrity and higher molecular weight, as assessed by the Agilent Technologies 4150 TapeStation Genomic DNA ScreenTape system.