Rabbit anti-GST Tag mAb

$187.11
In stock

SKU:  08L100035

Key features and details

This GST-Tag Rabbit monoclonal antibody offers high specificity and potency and consistency against the GST tag, which has been experimentally validated in Western blot (WB) and Immunoprecipitation (IP) assays.
Synonyms
GST; GST tag; GST-tag
Molecular Weight:
Observed at 27 KDa/ 33.9 KDa/ 50 KDa
Packaging size:
100 µL
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Product Description

MP Biomedicals GST-Tag Rabbit monoclonal antibody offers high specificity and potency and consistency against the GST tag, which has been experimentally validated in Western blotting (WB) and Immunoprecipitation (IP) assays.

Usage Statement

Research use only, not for direct human use.

Key Applications

Western blotting (WB), Immunoprecipitation (IP)

Specifications
SKU 08L100035
Alternate Names GST; GST tag; GST-tag
Antibody Type Monoclonal Antibody
Background_information Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions. A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Bioreactivity This GST-Tag Rabbit monoclonal antibody offers high specificity and potency and consistency against the GST tag, which has been experimentally validated in Western blot (WB) and Immunoprecipitation (IP) assays.
Concentration Please check the vial’s label
Conjugate Unconjugated
Formulation Details In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Grade Molecular Grade
Host Rabbit
Immunogen Immunogen is a full-length recombinant protein of GST protein.
Isotype IgG
Molecular Weight Observed at 27 KDa/ 33.9 KDa/ 50 KDa
Pack Size 100 µL
Preparation Method Affinity purification
Solubility Dissolves in Tris or PBS buffer immediately
Specificity High specificity against antigen GST tag
Storage -20°
Typical Working Concentration WB: 1:500 - 1:2000; IP: 1:50 - 1:200
Usage Statement Research use only, not for direct human use.
Documents

Certificate of Analysis (COA)

Datasheet

Brochures

Brochures
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