Product Description
MP Biomedicals GST-Tag Rabbit monoclonal antibody offers high specificity and potency and consistency against the GST tag, which has been experimentally validated in Western blotting (WB) and Immunoprecipitation (IP) assays.
Usage Statement
Research use only, not for direct human use.
Key Applications
Western blotting (WB), Immunoprecipitation (IP)
Specifications
| SKU | 08L100035 |
| Alternate Names | GST; GST tag; GST-tag |
| Antibody Type | Monoclonal Antibody |
| Background_information | Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions. A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. |
| Bioreactivity | This GST-Tag Rabbit monoclonal antibody offers high specificity and potency and consistency against the GST tag, which has been experimentally validated in Western blot (WB) and Immunoprecipitation (IP) assays. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Formulation Details | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
| Grade | Molecular Grade |
| Host | Rabbit |
| Immunogen | Immunogen is a full-length recombinant protein of GST protein. |
| Isotype | IgG |
| Molecular Weight | Observed at 27 KDa/ 33.9 KDa/ 50 KDa |
| Pack Size | 100 µL |
| Preparation Method | Affinity purification |
| Solubility | Dissolves in Tris or PBS buffer immediately |
| Specificity | High specificity against antigen GST tag |
| Storage | -20° |
| Typical Working Concentration | WB: 1:500 - 1:2000; IP: 1:50 - 1:200 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Brochures
Brochures
English (United States)