Rabbit anti GFP-Tag mAb

Name: Rabbit anti GFP-Tag mAb
SKU: 08L100036
Price: 187.11 USD

$187.11

In stock

SKU: 08L100036

Key features and details

This Rabbit anti-GFP-Tag mAb offers high specificity and potency and consistency against the GFP tag, which has been experimentally validated in Western blotting (WB) and Immunofluorescence (IF) assays.

Synonyms

GFP; GFP tag; GFP-tag

Molecular Weight:

Observed at 25KDa

Packaging size:

100 µL

Qty

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Product Description

MP Biomedicals Rabbit anti-GFP-Tag monoclonal antibody offers high specificity and potency and consistency against GFP tag, which has been experimentally validated in Western blotting (WB) and Immunofluorescence (IF) assays.

Usage Statement

Research use only, not for direct human use.

Key Applications

Western blotting (WB), Immunofluorescence (IF)

Specifications

SKU	08L100036
Alternate Names	GFP; GFP tag; GFP-tag
Antibody Type	Monoclonal Antibody
Background_information	The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells. Scientists Roger Y. Tsien, Osamu Shimomura, and Martin Chalfie were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.
Base Catalog Number	L100036
Bioreactivity	This Rabbit anti-GFP-Tag mAb offers high specificity and potency and consistency against the GFP tag, which has been experimentally validated in Western blotting (WB) and Immunofluorescence (IF) assays.
Concentration	Please check the vial’s label
Conjugate	Unconjugated
Formulation Details	In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Grade	Molecular Grade
Host	Rabbit
Immunogen	Immunogen is a full-length recombinant GFP protein.
Isotype	IgG
Molecular Weight	Observed at 25KDa
Pack Size	100 µL
Preparation Method	Affinity purification
Solubility	Dissolves in Tris or PBS buffer immediately
Specificity	High specificity against antigen tag
Storage	-20°
Typical Working Concentration	WB: 1:500 - 1:2000; IF: 1:50 - 1:200
Uniprot Number	P42212
Usage Statement	Research use only, not for direct human use.