Product Description
Pyruvate Kinase
Application Notes
Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Signalling
Specifications
| SKU | 0215199905 |
| Alternate Names | PK; ATP : pyruvate 2-O-phosphotransferase; Phosphoenolpyruvate kinase |
| Applications | Cell Signalling |
| Application Notes | Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP. |
| Base Catalog Number | 151999 |
| Biochemical Physiological Actions | Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP. |
| CAS # | 9001-59-6 |
| EC Number | 232-616-2 |
| Extinction Coefficient | 5.4 (280 nm) (Lit.) |
| Isoelectric Point | 7.6 |
| Molecular Weight | 237,000 |
| Pack Size | 5 KU |
| Personal Protective Equipment | Eyeshields, Gloves, Repirator Filter |
| Solubility | Dissolves readily at 5 mg/mL in 0.06M Tris/HCI pH 7.4 to give a clear colorless solution. |
| Source | Rabbit Muscle |
| Specific Activity | ≥200 u/mg protein |
| Storage | -20 °C |
| Unit Definition | The amount of enzyme which produces one micromole of pyruvate per minute at pH 7.6 from PEP in the presence of ADP at 30 °C |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
Powered by Bioz