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Isopropyl-β-D-thiogalactopyranoside, molecular biology, ≥99%

Isopropyl-β-D-thiogalactopyranoside, molecular biology, ≥99%

Synonyms IPTG; Isopropyl β-D-thiogalactoside; l-Propyl-b-D-thiogalactopyranoside

CAS Number: 367-93-1 Molecular Formula: C9H18O5S Molecular Weight: 238.298 g/mol

Beilstein Registry Number: 4631 EC Number: 206-703-0 MDL Number: MFCD00063273

SKU 02194029-CF
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Description

Product Description

Isopropyl-Β-D-Thiogalactopyranoside

Application Notes

Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

β-Galactoside inducer; protein expression

Specifications
SKU 02194029-CF
Alternate Names IPTG; Isopropyl β-D-thiogalactoside; l-Propyl-b-D-thiogalactopyranoside
Application Notes Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Base Catalog Number 194029
Beilstein Registry Number 4631
CAS # 367-93-1
EC Number 206-703-0
Grade Molecular Biology Grade
Hazard Statements H319-H351
Melting Point 121 °C
Molecular Formula C9H18O5S
Molecular Weight 238.298 g/mol
Optical Rotation (c=1, water)-26 ° to -33 °
Purity ≥99%
Safety Symbol GHS07,GHS08
Sterilization of Solutions Solutions can be sterile filtered using a 0.2 mm filter.
Typical Working Concentration The final concentration typically used in indicator plates is 0.2 mM.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.