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Insulin, human recombinant
Insulin, Human Recombinant
Methods for immobilizing insulin on polystyrene dishes have been reported for applications involving protein-free cell cultures.
|Alternate Names||Insulin sodium salt human; rh-Insulin|
|Application Notes||Methods for immobilizing insulin on polystyrene dishes have been reported for applications involving protein-free cell cultures.|
|Base Catalog Number||193900|
|Biochemical Physiological Actions||Two-chain polypeptide hormone produced by the ß-cells of pancreatic islets. Its molecular weight is ~5800 Da. The a and ß chains are joined by two interchain disulfide bonds. The a chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.|
|Extinction Coefficient||EM (278 nm): 6080 (33 mM phosphate, pH 7.0, bovine and porcine) (Lit.)|
|Isoelectric Point||~5.3 (for the native protein)(Lit.)|
|Melting Point||81 °C|
|Molecular Weight||5807.629 g/mol|
|Specific Activity||~28 IU/mg|
|Typical Working Concentration||1 to 10 ug/mL for cell culture|
|Unit Definition||1 unit corresponds to the efficiency of 0.04167 mg international standard substance. An HPLC method exists to test the potency.|