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Hygromycin B, solution

Hygromycin B, solution

Synonyms Antihelmycin; Hygromix-8

CAS Number: 31282-04-9 Molecular Formula: C20H37N3O13

Beilstein Registry Number: 6755837 EC Number: 250-545-5 MDL Number: MFCD06795479

SKU 02157513-CF
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Description

Product Description

Hygromycin B Solution

Application Notes

Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is used in gene transfer experiments as a selection agent.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antibacterial and antifungal agent

Specifications
SKU 02157513-CF
Alternate Names Antihelmycin; Hygromix-8
Application Notes Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is used in gene transfer experiments as a selection agent.
Base Catalog Number 157513
Beilstein Registry Number 6755837
Biochemical Physiological Actions Antibacterial and antifungal. Mode of action: Inhibition of protein synthesis, it interfere with translocation of the 70S ribosome, by inducing the misreading of the m-RNA template in prokaryotes and eukaryotes. It selectively penetrates cells that have been rendered permeable by virus infection.
CAS # 31282-04-9
EC Number 250-545-5
Format Solution
Formulation Details Hygromycin B is provided as a caramel-colored solution in water. Not prepared under sterile conditions. If a sterile solution is desired; working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use. Do not autoclave solutions of Hygromycin B.
Hazard Statements H301 + H311-H318-H330-H334
Molecular Formula C20H37N3O13
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
pKa 7.1 and 8.8(Lit.)
Purity ≥80%
RTECS Number WK2130000
Safety Symbol GHS05, GHS06, GHS08
Specific Activity ~450,000 u/mL
Sterilization of Solutions Solutions should be sterilized by means of a 0.2 micron filter. Do not autoclave solutions.
Typical Working Concentration For use as a selective agent, the suggested concentration range is 100- 800 μg/mL
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.