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Hygromycin B, phytopure™

Hygromycin B, phytopure™

$31.45
CAS Number: 31282-04-9

SKU: 02194170-CF

Synonyms
O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine
CAS Number:
31282-04-9
Molecular Formula:
C20H37N3O13
Molecular Weight:
527.50
EC Number:
250-545-5
MDL Number:
MFCD06795479
Product Description

An aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.

Application Notes

Hygromycin B is used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

In gene transfer experiments as a selection agent.

Specifications
SKU 02194170-CF
Alternate Names O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine
Application Notes Hygromycin B is used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.
Base Catalog Number 194170
Biochemical Physiological Actions Blocks polypeptide synthesis and inhibits elongation. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template.
CAS # 31282-04-9
EC Number 250-545-5
Format Powder
Hazard Statements H300 +H310 + H330-H318-H334
Molecular Formula C20H37N3O13
Molecular Weight 527.50
pKa 7.1
Purity 95%
RTECS Number WK2130000
Safety Symbol GHS05, GHS06, GHS08
Source Streptomyces hygroscopicus
Specific Activity >1000 u/mg
Typical Working Concentration When using this product for mammalian cell selection the working concentration is generally between 50 mg/mL and 1 mg/mL, but at times may go as high as 1 mg/mL. The appropriate concentration for use must be determined empirically for each cell type.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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