Human TNF-alpha ELISA Kit

TNF-alpha is the prototypic ligand of the TNF superfamily which plays a central role in inflammation, apoptosis, proliferation, invasion, angiogenesis, metastasis and morphogenesis. It is expressed on macrophages, endothelial, epithelial and tumor cells as a 26kDa transmembrane protein.

TNF-alpha is cleaved by proteolytic processing into six chains:

1. TNF membrane form
2. Intracellular domain 1
3. Intracellular domain 2
4. C-domain 1
5. C-domain 2
6. TNF soluble form

Signaling from TNF-alpha differs depending on the type of ligand initiating the signaling event (intracellular, membrane or soluble). As an example, the membrane form of TNF-alpha appears to mediate anti-tumorigenic therapeutic responses whereas the soluble ligand is linked to inflammation and proliferation.

In this ELISA kit, a TNF-alpha specific antibody has been pre-coated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an TNF-alpha specific biotinylated detection antibody is added and followed by washing with wash buffer. Afterwards, Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF-alpha captured in the plate.

Application Notes

Human TNF-alpha ELISA Kit (SKU: 08L100043) has been used to quantitatively measure the levels of TNF alpha in samples of biological samples through an enzyme-linked immunosorbent assay in a 96-well plate.

The detection range is 15.625-1000 pg/mL with a sensitivity of 9.375 pg/mL in ELISA assays.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

ELISA