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HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. It is used in a protein deposition technique in electron microscopy because it did not affect metal substrates.
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|Alternate Names||4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt; sodium N-(2-hydroxyethyl) piperazine-N'- (2-ethanesulfonate); sodium 4-(2-hydroxyethyl)-1- piperazineethanesulfonate|
|Application Notes||HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. It is used in a protein deposition technique in electron microscopy because it did not affect metal substrates.|
|Base Catalog Number||194828|
|Grade||Molecular Biology Grade|
|Personal Protective Equipment||Eyeshields, Gloves, Respirator filter|
|pH||9.5 - 10.8 (1% aq soln); Useful pH range=6.8-8.2(Lit)|
|pKa||7.55 at 25 °C (Lit.)|
|Solubility||Soluble in water.|
|Sterilization of Solutions||Solutions may be autoclaved under standard conditions|