Hepes
Application Notes
HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Buffer
| SKU | 02101926-CF |
| Alternate Names | 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) |
| Application Notes | HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures. |
| Base Catalog Number | 101926 |
| Beilstein Registry Number | 883043 |
| CAS # | 7365-45-9 |
| EC Number | 230-907-9 |
| Format | Crystalline, Free Acid |
| Hazard Statements | H317 |
| Molecular Formula | C8H18N2O4S |
| Molecular Weight | 238.302 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| pKa | 7.55 at 25 °C (Lit.) |
| Purity | ≥98% |
| RTECS Number | TL6809000 |
| Solubility | 5.25e+01 g/l |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | OD250 (10% aq soln) <0.2 , OD260 (10% aq soln) <0.1 and OD280 (10% aq soln) <0.1 |
![MP COA [101926] lot ID: M6876](https://www.mpbio.com/static/version1742546773/frontend/Mpbio/default/en_US/images/document.png "MP COA [101926] lot ID: M6876")