Guanidine Hydrochloride
Application Notes
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Chaotropic agent
| SKU | 04820539 |
| Alternate Names | Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride |
| Application Notes | Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions. |
| Base Catalog Number | 820539 |
| Beilstein Registry Number | 3591990 |
| CAS # | 50-01-1 |
| Density | 1.3 g/cm³ |
| EC Number | 200-002-3 |
| Format | Crystalline powder |
| Grade | Ultra Pure |
| Melting Point | 178-185°C |
| Molecular Formula | CH5N3·HCl |
| Molecular Weight | 95.53 g/mol |
| Pack Size | 500 g |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| pKa | 13.6 (Lit.) |
| Purity | ≥99% |
| RTECS Number | MF4300000 |
| Safety Symbol | GHS07 |
| Solubility | in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, g/100ml at 20°C: 215 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | OD260: <0.03 (6.0 M aq soln) |
