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Guanidine hydrochloride, optical density grade, ~99%

Guanidine hydrochloride, optical density grade, ~99%

Synonyms Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride; Guanidine HCl

CAS Number: 50-01-1 Molecular Formula: CH5N3·HCl Molecular Weight: 95.53 g/mol

Beilstein Registry Number: 3591990 EC Number: 200-002-3 MDL Number: MFCD00013026

SKU 02151210-CF
$23.90
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Description

Product Description

Guanidine Hydrochloride

Application Notes

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Strong denaturant

Specifications
SKU 02151210-CF
Alternate Names Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride; Guanidine HCl
Application Notes Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Base Catalog Number 151210
Beilstein Registry Number 3591990
CAS # 50-01-1
Density 1.3 g/cm³
EC Number 200-002-3
Format Crystalline powder
Grade Optical Grade
Hazard Statements H302-H315-H319
Melting Point 178-185°C
Molecular Formula CH5N3·HCl
Molecular Weight 95.53 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
pKa 13.6 (Lit.)
Purity 99%
RTECS Number MF4300000
Safety Symbol GHS07
Solubility in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, g/100ml at 20°C: 215
Typical Working Concentration Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance OD260 (6.0 M aq soln): ≤0.03