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Fluorescamine

Fluorescamine

Synonyms 4-Phenyl spiro-[furan-2(3H)-1'-(3'-H)-Isobenzofuran]-3,3'-dione; 4-Phenyl spiro-[furan-2(3H), 1-phthalan]-3,3'-dione

CAS Number: 38183-12-9 Molecular Formula: C17H10O4 Molecular Weight: 278.263 g/mol

Beilstein Registry Number: 921143 EC Number: 253-814-5 MDL Number: MFCD00005928

SKU 02195183-CF
$39.20
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Description

Product Description

Fluorescamine

Application Notes

Fluorescamine is a reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. It is also used for fluorometric assays of some proteolytic enzymes. it effectively blocks newly generated amino termini in protein sequence analyses by the Beckman automated sequencer. This product has been used in labeling casein so that it can be used as a substrate for measuring protease activity. Fluorescamine is used in many sensitive detection methods, eg. characterization of poly-L-lysine (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer, spectrofluorimetric analysis of procaine, detection and quantitation of residual aminopenicillins by HPLC after fluorescamine derivation, determination of lisinopril in human plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Fluorometric assay

Specifications
SKU 02195183-CF
Alternate Names 4-Phenyl spiro-[furan-2(3H)-1'-(3'-H)-Isobenzofuran]-3,3'-dione; 4-Phenyl spiro-[furan-2(3H), 1-phthalan]-3,3'-dione
Application Notes Fluorescamine is a reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. It is also used for fluorometric assays of some proteolytic enzymes. it effectively blocks newly generated amino termini in protein sequence analyses by the Beckman automated sequencer. This product has been used in labeling casein so that it can be used as a substrate for measuring protease activity. Fluorescamine is used in many sensitive detection methods, eg. characterization of poly-L-lysine (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer, spectrofluorimetric analysis of procaine, detection and quantitation of residual aminopenicillins by HPLC after fluorescamine derivation, determination of lisinopril in human plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.
Base Catalog Number 195183
Beilstein Registry Number 921143
CAS # 38183-12-9
EC Number 253-814-5
Format Powder
Molecular Formula C17H10O4
Molecular Weight 278.263 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.