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Ethidium bromide solution, 10 mL

Ethidium bromide solution, 10 mL

Synonyms 3,8-Diamino-5-ethyl-6-phenylphenanthridiniumbromide (6CI); Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide(8CI,9CI); 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide; 2,7-Diamino-9-phenylphenanthridine ethobromide; Dromilac; Homidium bromide

CAS Number: 1239-45-8 Molecular Formula: C21H20BrN3 Molecular Weight: 394.316 g/mol

Beilstein Registry Number: 3642536 EC Number: 214-984-6 MDL Number: MFCD00011724

Packaging size: 10 mL

SKU 04802511
$62.25
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Description

Product Description

Ethidium Bromide Solution

Application Notes

Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Electrophoresis;Staining

Specifications
SKU 04802511
Alternate Names 3,8-Diamino-5-ethyl-6-phenylphenanthridiniumbromide (6CI); Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide(8CI,9CI); 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide; 2,7-Diamino-9-phenylphenanthridine ethobromide; Dromilac; Homidium bromide
Application Notes Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Base Catalog Number 802511
Beilstein Registry Number 3642536
Biochemical Physiological Actions Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
CAS # 1239-45-8
Density 0.34 g/cm³
EC Number 214-984-6
Flash Point >100 °C
Format Solution
Melting Point 260-262 deg C (decomposes)
Molecular Formula C21H20BrN3
Molecular Weight 394.316 g/mol
Pack Size 10 mL
RTECS Number Ethidium Bromide SF7950000
Solubility Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a> is 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 2 mg/mL
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Vapor Pressure 1.2X10-12 mm Hg at 25 deg C /Estimated/