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Concanavalin A, FITC conjugated

Concanavalin A, FITC conjugated

$37.15
Concanavalin A, Fitc Conjugated

SKU: 02153245-CF

Synonyms
Con A
CAS Number:
Not applicable
Product Description

Concanavalin A, Fitc Conjugated

Application Notes

Concanavalin A used to examine tissue sections. It is conjugated to FITC, allowing fluorescent detection in binding assays for example detection of glycoproteins containing α−D-mannose or α−D-glucose. It is also used in blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, ymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Fluorescent detection in binding assays

Specifications
SKU 02153245-CF
Alternate Names Con A
Application Notes Concanavalin A used to examine tissue sections. It is conjugated to FITC, allowing fluorescent detection in binding assays for example detection of glycoproteins containing α−D-mannose or α−D-glucose. It is also used in blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, ymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.
Base Catalog Number 153245
Biochemical Physiological Actions Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
CAS # Not applicable
Conjugate FITC conjugate
Format Frozen Solution
Hazard Statements H317
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Preparation Method Gel filtration performed after conjugation to remove free FITC. Buffer: 0.05 M Tris- 0.15 M NaCl-0.004 M CaCl2, pH 7.0-7.2. contains sodium azide as a preservative.
Safety Symbol GHS07
Source Jackbean
Specificity (Carbohydrate) α-D-Mannose; α-D-Glucose; Branched mannose
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Datasheets

Datasheets
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United States)