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CHAPS

CHAPS

Synonyms 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] amino]-1-propanaminium inner salt

CAS Number: 75621-03-3 Molecular Formula: C32H58N2O7S Molecular Weight: 614.9

MDL Number: MFCD00012116

SKU 02190319-CF
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Description

Product Description

CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.

Application Notes

CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Detergent for solubilizing membrane proteins

Specifications
SKU 02190319-CF
Aggregation Number 4-14 (0-0.1 M Na+)
Alternate Names 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] amino]-1-propanaminium inner salt
Application Notes CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
Base Catalog Number 190319
CAS # 75621-03-3
Critical Micelle Concentration 6-10 mM
Hazard Statements H315-H319-H335-H360D
Melting Point 265°C (Lit.)
Molecular Formula C32H58N2O7S
Molecular Weight 614.9
pH 4.0 - 7.0 (10% aq soln)
Purity ≥98%
Safety Symbol GHS07, GHS08
Solubility Soluble in water (100 mg/mL - clear, colorless to slightly yellow solution).
Typical Working Concentration Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.