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Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.
Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.
|Alternate Names||Blue Bromophenol Sodium Salt; 3,3,5,5-Tetrabromophenolsulfophthalein sodium salt|
|Application Notes||Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.|
|Base Catalog Number||15250625|
|Beilstein Registry Number||6995701|
|Pack Size||25 g|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|Solubility||Soluble in water (30 mg/mL-clear, blueish violet solution) or ethanol (7 mg/mL).|
|UV Visible Absorbance||λ max 438 nm, λmax 591 nm (Lit.)|