Bovine Thrombin and Applications

Thrombin (factor IIa) is a serine protease that converts fibrinogen into fibrin in blood coagulation. With its procoagulant and anticoagulant functions, it plays a significant role in thrombosis and hemostasis. It is an agonist for many cellular responses during inflammation and wound repair. Many diseases including stroke and myocardial infarction involves thrombosis, and thrombin is therefore a preferred target of antithrombotic drugs.

Drugs available to block thrombin action include heparins, hirudins (lepirudin and bivalirudin), Vitamin K antagonists and a new generation of direct thrombin inhibitors, such as Dabigatran and Argatroban.

Thrombin is used throughout the diagnostics industry in a variety of coagulation assays, clotting factor tests and defibrination of blood or plasma for serum controls. It also finds applications in clot-activated blood collection tubes, in the meat industry as a meat glue and in bio-inks to produce engineered/artificial tissue using 3D printing. Thrombin is also used for site specific cleavage of recombinant fusion proteins and in biochemical and medical research applications.

The High Specific Activity Thrombin is especially suited for use in medical devices for haemostasis. A validated 2-step virus inactivated product is available for use in such applications.

MP Bio NZ manufactures Thrombin from bovine plasma using prothrombin activated with thromboplastin extracted from bovine lung tissue.

Types of Bovine Thrombin from MP Biomedicals New Zealand

NZ Bovine Thrombin, High Specific Activity (Cat.#02180539)

High specific activity Thrombin is lyophilized in glass vials under vacuum to optimize product performance and has a specific activity of > 2,000 NIH units/mg protein. High specific activity Thrombin is also used for site specific cleavage of recombinant fusion proteins and in biochemical and medical research applications, specifically in hemostasis applications. Bovine thrombin, high specific activity, is manufactured in New Zealand from bovine plasma of New Zealand origin using prothrombin activated with thromboplastin extracted from bovine lung tissue.

Manufactured in a state-of-the-art facility in New Zealand using a chromatographic extraction process, under Quality Systems that are ISO Certified.

NZ Bovine Thrombin, Low Specific Activity (Cat.# 02199907)

Thrombin is lyophilized in glass vials, under vacuum, up to a pack size of 500 KU. It is also available as a bulk lyophilized powder in customized pack sizes of 75 MU or higher.

The product has a Specific Activity of between 90 - 300 NIH units/mg protein and a total protein content of > 50%.

Product specifications

Test	Packaging*	pH	Excipients	% Protein	Thrombin Activity (Units / mg powder)	Thrombin Specific Activity(NIH Units / mg Protein)
High Specific Activity Thrombin (Cat.# 02180539)	Glass Vial	5.7 - 6.7	Sodium Citrate	Report the result	Report the result	> 2000
Low Specific Activity Thrombin (Cat.# 02199907)	Glass Vial	7.0 - 8.0	For Information Only (contains Tris, HCl, NaCl)	≥ 50%	Report the result	90 – 300

*Low Specific Activity Thrombin is also available as a bulk lyophilized powder in customized pack sizes of 75 MU or higher.

MP Bio New Zealand Proteins are Chromatographically Extracted from Bovine Plasma under Quality Systems that are ISO Certified.

All products are manufactured using New Zealand sourced raw materials. Documentation standards at MP Bio NZ are of the highest order and can support the exacting standards that are often sought by the pharmaceutical industry, which operates in a highly regulated environment.

MP Biomedicals has an ideal option for your requirements. Contact us to learn more about our full array of services.

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