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3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L

3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L

Synonyms TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

CAS Number: 54827-17-7 Molecular Weight: 240.35 g/mol

Packaging size: 1 L

SKU 0219602591
$694.30
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Description

Product Description

3,3,5,5-Tetramethylbenzidine Liquid Substrate

Application Notes

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Substrate for horseradish peroxidase

Specifications
SKU 0219602591
Alternate Names TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
Application Notes TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Base Catalog Number 2196025
CAS # 54827-17-7
Format Ready-to-use solution, Stabilized
Hazard Statements H226-H302-H312-H332-H370
Melting Point 334 to 336° F (NTP, 1992)
Molecular Weight 240.35 g/mol
Pack Size 1 L
Safety Symbol GHS02, GHS07, GHS08
Solubility less than 1 mg/mL at 68° F (NTP, 1992)
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.