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Complete Supplement Mixture (CSM) is a culture growth media supplement for virtually all strains of S. cerevisiae.
Complete Supplement Mixture (CSM) is a time-tested dropout supplement for virtually all strains of S. cerevisiae. Cells grow vigorously in CSM supplemented medium which is easily made by adding the CSM powder to DOB of DOBA Base Medium and autoclaving.
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Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Complete Supplement Mixture|
|Application Notes||Complete Supplement Mixture (CSM) is a time-tested dropout supplement for virtually all strains of S. cerevisiae. Cells grow vigorously in CSM supplemented medium which is easily made by adding the CSM powder to DOB of DOBA Base Medium and autoclaving.|
|Base Catalog Number||4500012|
|Formulation Details||Contents per Liter: Adenine 10* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 20 mg |L-Isoleucine 50 mg |L-Leucine 100 mg |L-Lysine HCl 50mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 50 mg |Uracil 20 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 790.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out.|
|Grade||Molecular Biology Certified™|
|Media Type||Yeast CSM|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM or CSM-Dropout formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.79g of CSM or the appropriate weight of CSM-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.|
|Solubility||Soluble in water.|
|Sterilization of Solutions||Autoclave at 121 °C for 15 minutes|
|Typical Working Concentration||0.790 g/L|