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Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.
Terrific Broth, modified has been formulated for the cultivation of molecular genetic strains of Escherichia coli and increased plasmid yield.
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Bacteria Cell Culture | Cultivation of molecular genetic strains of Escherichia coli and increased plasmid yield.
|Alternate Names||TB modified|
|Application Notes||Terrific Broth, modified has been formulated for the cultivation of molecular genetic strains of Escherichia coli and increased plasmid yield.|
|Base Catalog Number||1010417|
|Formulation Details||Powder | Following is composition of Terrific Broth; Modified in g/L: Tryptone - 12.0 g; Yeast extract - 24.0 g; Monopotassium phosphate - 2.2 g; Dipotassium phosphate - 9.4 g|
|Media Type||Terrific broth|
|Pack Size||500 g|
|pH||7.2 ± 0.2 (3.5% solution)|
|Preparation Method||Dissolve 47.6 g of the medium in 900 mL of deionized or distillled water. Add 8 mL of 50% glycerol. Mix well until evenly dispersed. Heat with frequent agitation and boil for one minute. Adjust final volume to 1000 mL. Sterilize in autoclave at 121 °C (15 lbs psi) for 15 minutes. Cool to room temperature. Store at 4 °C.|
|Sterility||Sterilize in autoclave at 121 °C (15 lbs psi) for 15 minutes.|