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Basal Medium Eagle (BME) is one of the most commonly used of all cell culture media. This medium is the result of numerous studies from the late 1950's analyzing the nutrient requirements and other components essential for the growth of cells in culture. Traditionally, BME has been used in studies to measure the growth response of normal (WI-38) and transformed (HeLa) cells in monolayer culture. BME may be used for a broad variety of cell lines when properly supplemented.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Bacteria Cell Culture, Mammalian Cell Culture
|Base Catalog Number||1003122|
|Formulation Details||Powder | Polypeptone 10.0; Biotryptase 10.0; Heart Peptone 3.0; Corn Starch 1.0; Sodium Chloride 5.0; Agar 13.5; Final pH 7.3 +/- 0.2|
|Media Type||Eagle, BME|
|Pack Size||10 L|
|pH||6.5 ± 0.5 (w/o sodium bicarb.), 7.3 ± 0.5 (adjusted with sodium bicarb.)|
|Preparation Method||Suspend 42.5 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Dispense and sterilize in an autoclave at 121 °C (15 lbs. psi) for 15 minutes. Cool to 45-50 °C and add 5-10% sterile defibrinated sheep or rabbit blood if desired.|
|Usage Statement||Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.|