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Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine w/o sodium carbonate, 10 L

Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine w/o sodium carbonate, 10 L

$27.10
Basal Medium Eagle (BME) is one of the most commonly used ynthetic basal medium for supporting the growth of many different mammalian cells

SKU: 091003122

Packaging size:
10 L
Product Description

Basal Medium Eagle (BME) is one of the most commonly used of all cell culture media. This medium is the result of numerous studies from the late 1950's analyzing the nutrient requirements and other components essential for the growth of cells in culture. Traditionally, BME has been used in studies to measure the growth response of normal (WI-38) and transformed (HeLa) cells in monolayer culture. BME may be used for a broad variety of cell lines when properly supplemented.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Bacteria Cell Culture, Mammalian Cell Culture

Specifications
SKU 091003122
Base Catalog Number 1003122
Format Solution
Formulation Details Powder | Polypeptone 10.0; Biotryptase 10.0; Heart Peptone 3.0; Corn Starch 1.0; Sodium Chloride 5.0; Agar 13.5; Final pH 7.3 +/- 0.2
Media Type Eagle, BME
Pack Size 10 L
pH 6.5 ± 0.5 (w/o sodium bicarb.), 7.3 ± 0.5 (adjusted with sodium bicarb.)
Preparation Method Suspend 42.5 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Dispense and sterilize in an autoclave at 121 °C (15 lbs. psi) for 15 minutes. Cool to 45-50 °C and add 5-10% sterile defibrinated sheep or rabbit blood if desired.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United States)

Datasheets

Datasheets
English (United States)