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Anti-ß-galactosidase, purified mouse monoclonal antibody, 100 µg

Anti-ß-galactosidase, purified mouse monoclonal antibody, 100 µg

MDL Number: MFCD00162155

Packaging size: 100 µg

SKU 08633651
$197.15
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Description

Product Description

Anti-Galactosidase, purified monoclonal antibody [subclass IgG2a(k)] was purified from ascites of a mouse hybridoma and recognizes E. coli β-galactosidase. Recognizes the native and denatured enzyme near the C-terminal end.

Application Notes

Monoclonal anti-β-galactosidase antibodies may be used in dot blot, western blot and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the step-wise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Dot Blot | Immunoblot | Immunocytochemistry | Immunocytochemistry | Immunoblot | Dot blot

Specifications
SKU 08633651
Antibody Type Monoclonal Antibody
Application Notes Monoclonal anti-β-galactosidase antibodies may be used in dot blot, western blot and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the step-wise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.
Base Catalog Number 633651
Clone Name 6A-11
Concentration 2.2 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Immunogen Betagalactosidase from E. coli
Isotype IgG2a, kappa
Pack Size 100 µg
Purity Immunoaffinity Chromatography 
Solubility Soluble in water
Species Reactivity Bacteria
Specificity The monoclonal antibody recognizes E. coli b-Galactosidase. In a dot blot assay the antibody reacts with both native and denatured b-Galactosidase.
Typical Working Concentration Dilutions:Tissue Staining: 1:5 - 1:20 Immunoblotting: 1:10 - 1:50 Elisa/FIA: 1:40 - 1:200 Dot Blot: 1:5000 Immunoprecipitation: 25 mg/ml
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.