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Anti-rat IgG (whole molecule) goat affinity-purified antibody, 2 mg

Anti-rat IgG (whole molecule) goat affinity-purified antibody, 2 mg

Packaging size: 2 mg

SKU 0855740
$159.60
In Stock
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Description

Product Description

Goat affinity purified antibody to rat IgG (whole molecule) is goat affinity purified antibody to rat IgG (whole molecule) and buffer salts.

Application Notes

Goat affinity purified antibody to rat IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry | ELISA

Specifications
SKU 0855740
Antibody Type Polyclonal Antibody
Application Notes Goat affinity purified antibody to rat IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number 55740
Concentration Total Protein: 1.02 mg/mL Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Goat
Pack Size 2 mg
Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Rat (Whole Molecule)
Specificity It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration SUGGESTED WORKING DILUTIONS 1:50 - 1:200 for Tissue Staining 1:100 - 1:500 for Immunoblotting 1:400 - 1:2,000 for Elisa
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.