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Anti-mouse IgG (whole molecule) goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-mouse IgG (whole molecule) goat IgG fraction, fluorescein-conjugated, 2 mL

MDL Number: MFCD00162642

Packaging size: 2 mL

SKU 0855493
$276.15
In Stock
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Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat IgG Fraction to Mouse IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855493
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat IgG Fraction to Mouse IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Base Catalog Number 55493
Concentration Total Protein: 24.4 mg/mL; Antibody Protein: 10 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Mouse IgG
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity Purified
Solubility Soluble in water
Species Reactivity Mouse (Whole Molecule)
Specificity This product shows reactivity to mouse IgG; cross-reactivity to other species may exist.
Typical Working Concentration Anti-Mouse IgG (whole molecule)-FITC antibody is suitable for use in immunohistochemistry (1:200) and immunofluorescence (1:100).
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.