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Anti-human fibrinogen goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-human fibrinogen goat IgG fraction, fluorescein-conjugated, 2 mL

Packaging size: 2 mL

SKU 0855169
$305.00
In Stock
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Description

Product Description

Fluorescein-conjugated Goat IgG Fraction to Human Fibrinogen is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human fibrinogen and buffer salts.

Application Notes

Fluorescein-conjugated Goat IgG Fraction to Human Fibrinogen is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855169
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-conjugated Goat IgG Fraction to Human Fibrinogen is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number 55169
Concentration Total Protein: 16.8 mg/mL; Antibody Protein: 3.5 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Purified fibrinogen derived from pooled human plasma
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human, IgG Fraction
Specificity This product shows reactivity to human fibrinogen; cross-reactivity to other species may exist. Antibody activity to serum proteins is not present.
Typical Working Concentration Dilution Recommendations: 1. Incident light excitation-Direct immunofluorescence: 1/10 - 1/80; Indirect immunofluorescence: 1/20 - 1/400. 2. Transmitted light excitation-Direct immunofluorescence: 1/5 - 1/40; Indirect immunofluorescence: 1/10 - 1/80; 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Uniprot Number P02671
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.